实验室承担的在研科研项目60余项，其中包括国家科技部 “973” 项目，“863”项目和“十五攻关”项目、国家自然科学基金重点及面上项目、国家教育部科技重点项目、省级科研项目、对外协作科研项目等，近五年实到科研经费2000多万元。实验室近五年在JBC、Nucleic Acids Research、JMB和Biochemistry等国际重要学术刊物上共发表论文128篇，其中SCI检索杂志论文86篇(累计影响因子近170)，影响因子3以上的论文超过20篇；主编专著四部和研究生教材一部。获省、部级一等奖、二等奖各一项。(其中：曹淑桂 酶催化有机合成和高分子合成的研究 吉林省科技进步奖二等奖 2000;
曹淑桂，张今，张学忠，冯雁，李正强等，酶分子工程及应用基础研究（ 6210901），吉林省科技进步奖一等奖，2002。)

专利成果
1． 含锌分子筛抗菌材料及其制备方法 李正强，阎雁，肖丰收
专利号：ZL 03 1 27125.1 (中国发明专利)
2. 具有流动相的凝胶介质制备型电泳装置 李正强，张华利，程瑛琨，刘彬，王雪丽
专利号：ZL 2003 1 0116010.3 (中国发明专利)
3. 玉米肽、制备方法及其应用 张学忠，徐力，吴晓霞，侯瑞珍，王华，黄宜兵
专利号：ZL 2003 1 0115958.7 (中国发明专利)
4. 大豆肽、制备方法及其应用 张学忠，吴晓霞，徐 力，黄宜兵
专利号：ZL 2004 1 0010997.5 (中国发明专利)
5. 环糊精衍生物的药物用途及其药物组合物 罗贵民，阎岗林，贾志丹，牟颖，任晓君，
刘俊秋，张鲲，沈家骢
专利号：ZL03 1 19443.5 (中国发明专利)
6. 环糊精衍生物的药物用途及其药物组合物 　罗贵民，阎岗林，贾志丹，牟颖
专利号：ZL 03119443.5 (中国发明专利)
7. 环糊精衍生物在饮料中的应用 阎岗林，罗贵民，牟颖，贾志丹
专利号：ZL 03119442.7 (中国发明专利)
8. 微流控芯片流式生化分析仪及检测生化组分的方法 牟颖，金钦汉，杨蕊，吴喆
专利号：ZL 03127188 (中国发明专利)
9. 化学突变具有底物结合部位的单克隆抗体制备含硒抗体酶 　罗贵民、朱振齐、丁兰、孙启按、
高贵、刘仔、扬同书、沈家骢
专利号 ： ZL94102481.4 (中国发明专利)
10. 含硒环糊精及其在制备治疗白内障药物中的应用 罗贵民、刘俊秋、任晓君、阎岗林、牟颖、
杨同书、沈家骢
专利号 ： ZL00133040.3 (中国发明专利)
11. 活力高于天然酶的含硒抗体酶的制备方法 丁兰、赵大庆、倪嘉缵、罗贵民、刘仔
专利号 ： ZL96112628.0 (中国发明专利)

　
2007年文章

1. Li YY, Wang R, Zhang GL, Zheng YJ, Zhu P, Zhang ZM, Fang XX, Feng Y*. An archaeal histone-like protein mediates efficient p53 gene transfer and facilitates its anti-cancer effect in vitro and in vivo. Cancer Gene Ther. 2007 Sep 14 IF=4.187

2. Guo Yi, Ma Yudan, Xu Li, Li Jun, and Yang Wensheng. Conformational Change Induced Reversible Assembly/Disassembly of Poly-L-lysine-Functionalized Gold Nanoparticles. J. Phys. Chem. C 2007, 111, 9172-9176 IF=4.115

3. Sheng Yongjie, Zeng Zhen, Peng Wei, Jiang Dazhi, Li Shuang, Sun Yanhong, Zhang Jin. Design and switch of catalytic activity with the DNAzyme-RNAzyme combination. FEBS LETTERS 2007,581 (9): 1763-1768 IF=3.415

4. Lv Shaowu, Wang Xiaoguang, Mu Ying, Zang Tianzhu, Ji Yuetong, Liu Junqiu, Shen Jiacong, Luo guimin. A novel dicyclodextrinyl diselenide compound with glutathione peroxxidase activity. FEBS Journal IF=3.033

5. Li Shuang, Li Bin, Fei Yanqun, Jiang Dazhi, Sheng Yongjie, Sun Yanhong, Zhang Jin. Exon grafting yields a “two active-site” lysozyme. Biochem. Biophy. Res. Commun. 2007, 358(4): 997-1001 IF=2.9

6. Yu Huijun, Ge Yan, Wang Ying, Lin Chi Tsai, Li Jing, Liu Xiaoman, Zang Tianzhu, Xu Jiayun, Liu Junqiu, Luo Guimin, Shen Jiacong. A fused selenium-containing protein with both GPx and SOD activities. Biochem. Biophy. Res. Commun.2007,358 (3): 873-878 IF=2.9

7. Ren Xiaodong, Yu Dawei, Yu Lei, Gao Gui, Han Siping, Feng Yan. A new study of cell disruption to release recombinant thermostable enzyme from Escherichia coli by thermolysis. JOURNAL OF BIOTECHNOLOGY 2007,129 (4): 668-673 IF=2.687

8. Wang Yuhua, Li Yan, Pei Xiaolin, Yu Lei, Feng Yan. Genome-shuffling improved acid tolerance and L-lactic acid volumetric productivity in Lactobacillus rhamnosus JOURNAL OF BIOTECHNOLOGY 2007,129 (3): 510-515 IF=2.687

9. Cai J, Wang Y, Liu D, Zeng Y, Xue Y, Ma Y, Feng Y*. Fervidobacterium changbaicum sp. nov., a novel thermophilic anaerobic bacterium isolated from a hot spring of the Changbai Mountains, China. Int J Syst Evol Microbiol.2007 Oct;57(Pt 10):2333-6. IF=2.662

10. Yu Dahai, Wang Zhi, Zhao Lifang, Cheng Yueming and Cao Shugui. Resolution of 2-octanol by SBA-15 immobilized Pseudomonas sp. Lipase, Journal of Molecular Catalysis B: Enzymatic, 2007,48(24):64-69 IF=2.149

11. Yu Dahai, Wang Zhi, Chen Peng, Jin Li, Cheng Yueming, Zhou Jianguang and Cao Shugui. Microwave-assisted resolution of (R,S)-2-octanol by enzymatic transesterification, Journal of Molecular Catalysis B: Enzymatic,200748(3):51-57 IF=2.149

12. Yu Dahai, Chen Peng, Wang Lei, Gu Qiang, Li Yan, Wang Zhi and Cao Shugui. A chemo-enzymatic process for sequential kinetic resolution of (R,S)-2-octanol under microwave irradiation. Process Biochemistry, 200742( 9):1312-1318 IF=2.008

13. Yu Dahai, Wang Lei, Gu Qiang, Chen Peng, Li Yan, Wang Zhi and Cao Shugui. A two-step enzymatic resolution of glycidyl butyrate. Process Biochemistry, 200742(9):1319-1325 IF=2.008

14. Li Hong-Mei, Guo Ping, Hu Xi, Xu Li and Zhang Xuezhong. Preparation of corn (Zea mays) peptides and their protective effect against alcohol-induced acute hepatic injury in NH mice Biotechnology and Applied Biochemistry 2007, 47(3), 169–174 IF=1.89

15. Ren Xiaodong, Yu Dawei, Han Siping, Feng Yan. Thermolysis of recombinant Escherichia coli for recovering a thermostable enzyme BIOCHEMICAL ENGINEERING JOURNAL 2007 ,33 (1): 94-98. IF=1.781

16. Yu, Dahai, Wang Zhi, Liu Yan, Jin Li, Cheng Yueming, Zhou Jianguang, Cao Shugui Quantum dot-based pH probe for quick study of enzyme reaction kinetics. ENZYME AND MICROBIAL TECHNOLOGY 2007 ,41 (1-2): 127-132 IF=1.705

17. Kang Bo, Huang WeiMin, Zhang YingJiu, Zhang XueNa, Lin HaiBo.Studies on the 4-amino-dimethyl-aniline hydrochloride degraded by a bio-electro reactor. Chem. Res. Chinese U. 2007,28 (3): 556-558 IF=0.771

18. Lin Feng, Li Ying, ang Wen kui, Liang Bing, Mu Ying, Sun Ye, Li Wei, Luo Guimin. Rapid selection of phage Se-scFv with GPX activity via combination of phage display antibody library with chemical modificationv.Chem. Res. Chinese U. 2007,23 (1): 58-63 IF=0.411

19. Zhang Guirong, Gao Renjun, Zhang Aijun,Rao Lang,Cao Shugui.High-throughput screening of the enantioselectivity of hyperthermophilic mutant esterases from Archaeon Aeropyrum pernix K1 for resolution of (R, S)-2-octanol acetate. Chem. Res. Chinese U. 2007,23 (3): 319-324 IF=0.411

20. Gao Gui, Zheng Liangyu, Zhang Zuoming, Han Siping, Cao Suigui. Monitoring the hydrolysis of olive oil catalyzed by lipase via acid value detection. Chem. Res. Chinese U. 2007,23 (2): 196-199 IF=0.411

21. He Lingbing, Wang Yan, Wang Ge, Chen Chao, Cao Shugui.Expression and purification of active recombinant human nerve growth factor from Escherichia coli.C Chem. Res. Chinese U. 2007,23 (2): 237-240 IF=0.411

22. Du Xiubo, Sun Ye, Lin Feng, Zheng Keyan, Wang Kewei, Lin Tingting, Liu Junqiu, Shen Jiacong, Luo Guimin. Chemical chaperones increasing expression level of soluble single-chain Fv antibody(scFv2F3). Chem. Res. Chinese U. 200723 (1): 69-75 IF=0.411

23. Wang Hua, Zeng Hongbin, Yang Shaojian, Gao Shen, Huang Yibing, Hou Ruizhen, Zhao Mifeng, Xu Li and Zhang Xuezhong. Cellular Adhesion Tripeptide RGD Inhibits Growth of Human Ileocecal Adenocarcinoma Cells HCT-8 and Induces Apoptosis Chem. Res. Chinese U. 2007, 23(5): 1-4 IF=0.411

2006年文章

1. Xiaoyu Yang, Zhengqiang Li, Bin Liu, Achim Klein-Hofmann, Ge Tian, Yefei Feng, Yu Ding, Dangsheng Su, and Fengshou Xiao. “Fish-in-Net” Encapsulation of Enzymes in Macroporous Cages as Stable, Reusable, and Active Heterogeneous Biocatalysts. Adv.Mater. 2006,18,410-414 IF: 9.107

2. Jinsong Zhou, Dongyun Hao, Xudong Wang, Teimei Liu, Chengyan He, Feng Xie, Yanhong Sun and Jin Zhang, An important role of a “probable ATP-binding component of ABC transporter” during the process of pseudomonas aeruginosa resistance to fluoroquinolone, Proteomics 2006, 6, 2495-2503 IF:6.088

3. Qiuyan Wang, Guangyu Yang, Yanli Liu, and Yan Feng*. Discrimination of Esterase and Peptidase Activities of Acylaminoacyl Peptidase from Hyperthermophilic Aeropyrum pernix K1 by a Single Mutation. J. Biol. Chem., 2006, 281:18618-18625 IF:5.854

4. Kun Zhang, Tianzhu Zang, Wei Yang, Ye Sun, Ying Mu, Junqiu Liu, Jiacong Shen, Guimin Luo*. Single-chain antibody displays glutathione S-transferase activity. J. Bio. Chem. 2006, 281 (18): 12516-12520 IF=5.854

5. Guangwei Liu, Pingsheng Gong, Hongguang Zhao, Zhicheng Wang, Shouliang Gong* and Lu Cai. Effect of low-level radiation on the death of male germ cells. Radiat. Res. 2006,165 (4): 379-389 IF=3.099

6. Mingxiao Ma, Ningyi Jin, Zhenguo Wang, Ruilin Wang, Dongliang Fei, Min Zheng, Gefen Yin, Chang Li, Leili Jia, Koushi Jin, Yingjiu Zhang*. Construction and immunogenicity of recombinant fowlpox vaccines coexpressing HA of AIV H5N1 and chicken IL18. Vaccine 2006,24(20):4304-4311 IF=2.822

7. Jinghui Xi, Xu Wang, Shanyu Li, Xin Zhou, Lin Yue, Jia Fan and Dongyun Hao*. Polyethylene glycol fractionation improved detection of low-abundant proteins in two-dimensional electrophoresis analysis of plant proteome. Phytochemistry 2006, 67(21), 2341-2348 IF=2.78

8. Jingyan Wei*, Yang Liu , Songchuan Yang , Junjie Xu , Hangtian Kong , Bing Han , Yongli Bao , Yin Wu , Weitian Yin , Wei Li , Ganglin Yan ,Guimin Luo, Haopeng Xu, Yuxin Li ,Bai Yang . Screening of single-chain variable fragments against TSP50 from a phage display antibody library and their expression as soluble proteins. J. Biomol. Screen. 2006,11 (5): 546-552 IF=2.763

9. Yibing Huang, Yu Cai, Sen Yang, Hua Wang, Ruizhen Hou, Li Xu, Xiaoxia Wu and Xuezhong Zhang*. Synthesis of tetrapeptide Bz-RGDS-NH2 by a combination of chemical and enzymatic methods. J. Biotechnol. 2006,125(3):311-318 IF=2.687

10. Bo Zhao, Ningyi Jin, Ruilin Wang, Lishu Zhang, Yingjiu Zhang*. Immunization of a DNA Vaccine from SARS-CoV Spike Protein Fragment 1 in Mice. Viral Immunolo 2006,19(3): 518-524 IF=2.03

11. Rui Yang, Yuxi Yan, Ying Mu, Wei Ji, Xuwen Li, Mingqiang Zou, Qiang Fei, Qinhan Jin*. A rapid and facile method for hydrothermal synthesis of CdTe nanocrystals under mild conditions. J. Nanosci. Nanotechno.. 2006, 6 (1): 215-220 IF=1.932

12. Ruizhen Hou, Yan Yang, Gang Li, Yibing Huang, Hua Wang, Yunjia Liu, Li Xu, Xuezhong Zhang*. Synthesis of a Precursor Dipeptide of RGDS Catalyzed by Industrial Protease Alcalase. Biotechnol. Appl. Biochem. 2006,44(2):73-80 IF=1.89

13. Xiaodong Ren, Dawei Yu, Siping Han, Yan Feng*. Optimization of recombinant hyperthermophilic esterase production from agricultural waste using response surface methodology. Bioresource Technol. 2006,97(18),2345-2349 IF=1.863

14. Shuwen Guan, Lengxin Duan, Yuanyuan Li, Benxiang Wang, Qiuli Zhou*. A novel polypeptide from Cervus nippon Temminck proliferation of epidermal cells and NIH3T3 cell line. Acta Biochim. Pol. 2006,53 (2): 395-397 IF=1.863

15. Yibing Huang, Yu Cai, Sen Yang, Hua Wang, Ruizhen Hou, Li Xu, Xuezhong Zhang*. Chemo-enzymatic synthesis of precursor tetrapeptide Bz-RGDS-NH2 of cellular adhesion motif in low water organic media. Enzyme Microb.Technol. 2006,39(5): 1159-1165 IF=1.705

16. Guirong Zhang, Renjun Gao, Liangyu Zheng, Aijun Zhang, Yuanhong Wang, Qiuyan Wang, Yan Feng*, Shugui Cao*. Study on relationship between structure and enantioselectivity of a hyperthermophilic esterase from archaeon Aeropyrum pernix K1. J. Mol. Cata. B: Enzym. 2006, 38: 148-153 IF=1.685

17. Liangyu Zheng, Suoqin Zhang, Lifang Zhao, Guangshan Zhu, Xueying Yang, Gui Gao, Shugui Cao*. Resolution of N-(2-ethyl-6-methylphenyl)alanine via free and immobilized lipase from Pseudomonas cepacia. J. Mol. Cata. B: Enzym. 2006, 38: 119-125 IF=1.685

18. Liangyu Zheng, Suoqin Zhang, Xiaoqi Yu, Lifang Zhao, Gui Gao, Xueying Yang, Haifeng Duan, Shugui Cao. Enhancement of enantioselectivity in lipase-catalyzed resolution of N-(2-ethyl-6-methylphenyl)alanine by additives. J. Mol. Cata. B: Enzym. 2006, 38: 17-23 IF=1.685

19. Jisheng Ma, Zuoming Zhang, Baijing Wang, Xiangju Kong, Yuguo Wang, Shugui Cao*, Yan Feng*. Overexpression and characterization of a lipase from Bacillus subtilis. Protein Expres. Purif. 2006, 45:22–29 IF=1.553

20. Zhigang Wang, Shoufu Pan*, Minggang Yao, Gang Wang, Shaowu Lv, Jing Yang, Mingxing Jin, Weiqi Li. The two hydrogen transfer dissociation channels of nicotine molecule in the gas phase. J. Mol. Struct. 2006,767 (1-3): 95-101 IF=1.44

21. Liangyu Zheng, Souqin Zhang, Fang Wang, Gui Gao,and Shugui Cao*. Chemoenzymatic synthesis of the chiral herbicide: (S)-metolachlor. Can. J. Chem. 2006,84: 1058-1063 IF=1.118

22. Yaqiong Hao, Yuqing Wu*, Junqiu Liu*, Guimin Luo, Guangdi Yang. 1H NMR Study on the Inclusion Complex of Glutathione with a Glutathione Peroxidase Mimic, 2,2￠-ditelluro-bridged b-cyclodextrins. J. Incl. Phenom. Macro. Chem. 2006, 54:171–175 IF=1.02