王道文 分子农业生物学研究中心主任
王道文
职称：研究员
职务：分子农业生物学研究中心主任
联系方式: E-mail
1993年在英国John Innes研究中心获博士学位，1997年入选中国科学院"百人计划"， 现任分子农业生物学研究中心主任， 1997年获国家杰出青年科学基金。
王道文博士领导的创新研究组主要研究方向是植物分子和细胞生物学，农作物籽粒品质、养分利用效率以及抗病性的遗传基础、分子机制与改良。
研究内容包括以下三个方面
1. 小麦籽粒加工品质的遗传基础与改良
小麦籽粒品质性状包括多个方面，如蛋白品质、淀粉品质、以及营养品质等。本组目前的研究主要涉及小麦籽粒加工品质的遗传基础与改良，具体内容是从小麦及其近缘种中分离与小麦籽粒加工品质相关的储藏蛋白基因，研究其产物的功能，并在此基础上建立我国小麦加工品质改良的分子育种研究体系。本项研究由中科院生物技术基金、科技部973项目基金和科技部863项目基金资助。
2. 植物氮、磷元素利用效率的分子基础与改良
提高农作物对土壤中矿质元素的利用效率对于减少化肥施用量、降低农作物成本和保护生态环境具有重要意义。多年的遗传、生理和生化研究表明植物对矿质元素的吸收、同化和利用过程受多个基因的参与和调控。利用模式植物拟南芥开展了对植物核苷转运蛋白和植物紫色酸性磷酸酶的研究，目的是在认识功能的基础上，利用这两类基因分别提高农作物对氮、磷元素的利用效率。本项研究由科技部转基因植物专项经费和国家自然科学基金资助。
3.小麦细胞与黄矮病毒互作的分子机制及其在提高小麦抗病性研究中应用
黄矮病毒（BYDV）感染小麦后引起小麦植株严重黄化和矮化，创新研究组以BYDV的运动蛋白为起点开始探索BYDV的韧皮部局限性是否是导致病害症状的主要原因。研究结果表明BYDV MP具有较强的核酸结合性能。在昆虫和植物细胞中表达BYDV MP，发现MP蛋白不仅定位于细胞核膜，而且能在核膜上形成一种特殊的结构。基于上述认识，形成了继续研究BYDV MP与寄主细胞互作的工作模型。此研究得到国家杰出青年基金和科技部973项目资助。
主要论文：
小麦遗传学方面
Zhu YF, Li YW, Chen Y, Li H, Liang H, Yue SJ, Zhang AM, Zhang XQ, Wang D*, Jia X. Generation and characterization of a high molecular weight glutenin 1Bx14-deficient mutant in common wheat. Plant Breeding 2005; 124: 421-427.
Wan Y, Yan Z, Liu K, Zheng Y, D'Ovidio R, Shewry PR, Halford NG, Wang D. Comparative analysis of the D genome-encoded high-molecular weight subunits of glutenin. Theor Appl Genet 2005; 111: 1183-1190.
Li W, Wan Y, Liu Z, Liu K, Liu X, Li B, Li Z, Zhang X, Dong Y, Wang D*. Molecular characterization of HMW glutenin subunit allele 1Bx14: further insights in to the evolution of Glu-B1-1 alleles in wheat and related species. Theor Appl Genet 2004; 109: 1093-1104.
Bai J, Liu K, Jia X, Wang D*. An analysis of homoeologous microsatellites from Triticum urartu and Triticum monococcum. Plant Sci 2004; 166: 341-347.
Liu Z, Yan Z, Wan Y, Liu K, Zheng Y, Wang D*. Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species. Theor Appl Genet. 2003; 106: 1368-1378.
Wan Y, Wang D, Shewry R, Halford G. Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrica. Theor Appl Genet 2002; 104: 828-839.
Wan Y, Liu KF, Wang D*, Shewry PR. (2000) High molecular weight subunits in the Cylindropyrum and Vertebrata section of the Aegilops genus and identification of subunits related to those encoded by the Dx alleles of common wheat. Theor Appl Genet 2000; 101: 879-884.
植物分子生物学方面
Weiqiang Qian, Chunmei Yu, Huanju Qin, Xin Liu, Aimin Zhang, Ida Elisabeth Johansen, and Daowen Wang*. Molecular and functional analysis of phosphomannomutase (PMM) from higher plants and genetic evidence for the involvment of PMM in ascorbic acid biosynthesis in Arabidopsis and Nicotiana benthamiana. Plant Journal. January, 2007; doi:10.111/j.1365-313X.2006.02967.x.
Chen K, Xu M, Li G, Liang H, Xia Z, Liu X, Zhang J, Zhang A, Wang D*. Identification of AtENT3 as the main transporter for uridine uptake in Arabidopsis roots. Cell Res 2006; 16: 377-388.
Liu X, Liu X, Zhang J, Xia Z, Liu X, Qin H, Wang D*. Molecular and functional characterization of sulfiredoxin homologs from higher plants. Cell Res 2006; 16: 287-296.
Zhang J, Zhu H, Liang H, Liu K, Zhang A, Ling HQ, Wang D*. Further analysis of the function of AtBHLH29 in regulating the iron uptake process in Arabidopsis thaliana. Journal of Integrative Plant Biology 2006; 48: 75-84.
Yuan YX, Zhang J, Wang D, Ling HQ. AtbHLH29 of Arabidopsis thaliana is a functional ortholog of tomato FER involved in controlling iron acquisition in strategy I plants. Cell Res 2005; 15: 613-21.
Zhu H, Qian W, Lu X, Li D, Liu X, Liu K, Wang D*. Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower. Plant Mol Biol 2005; 59: 581-594.
Li G, Liu K, Baldwin S, Wang D*. Equilibrative nucleoside transporters of Arabidopsis thaliana: cDNA cloning, expression pattern and analysis of transport activities. J Biol Chem 2003; 278: 35732-35742.
Xie C, Zhang JS, Zhou HL, Li J, Zhang ZG, Wang D, Chen SY. Serine/threonine kinase activity in the putative histidine kinase-like ethylene receptor NTHK1 from tobacco. Plant J 2003; 33: 385-393.
Li D, Zhu H, Liu K, Leggewie G, Udvardi M, Wang D*. Purple acid phosphatases of Arabidopsis thaliana: comparative analysis and differential regulation by phosphate deprivation. J Biol Chem 2002; 277: 27772-27781.
Li J, Wang D*. Cloning and in vitro expression of the cDNA encoding a putative nucleoside transporter from Arabidopsis thaliana. Plant Sci 2000; 157: 23-32.
