杨维才 中国科学院分子发育生物学重点实验室主任
Email:wcyang@genetics.ac.cn
杨维才 男，博士，研究员，博士生导师。
1984年 兰州大学学士。
1987年 兰州大学硕士。
1994年 荷兰Wageningen大学博士。
1996-年 美国Cold Spring Harbor实验室博士后，
1996-2000年 新加坡国立大学分子农业生物学院博士后。
2000－2002年 新加坡国立大学分子农业生物学院高级科学家。植物生殖和棉花生物技术实验室主任。
2001年 中国科学院“百人计划”入选者。
主要研究方向
植物生殖的分子遗传学，尤其是雌配子体发育过程中细胞极性、细胞命运和细胞分化的遗传控制，其目标是利用拟南芥为模式植物来探讨基本发育生物学问题，并以此为模式研究农作物基因功能。
主要研究内容
1. 植物雌配子体发育
植物生殖生物学是一门传统学科，传统的研究主要集中于对生殖过程的结构,形态和组织胚胎学描述，由于方法和技术方面的局限,对其分子及遗传机理的研究进展非常缓慢。近年来由于Gene Trap和 Enhancer Trap等插入突变系统的建立和激光共聚焦显微技术的应用。植物生殖生物学的研究逐渐成为研究热点。本研究组研究的领域主要集中在植物雌配子体的发育方面,如孢原细胞和胚囊中卵细胞，中央细胞的命运决定机理等.通过对配子体突变体的遗传分析和基因克隆，了解植物生殖过程中的基因调控及分子机理。对一些基本的生物学现象，如细胞极性、细胞命运的确立及受精机理等进行更深入的研究，了解植物的生殖过程。
2．线粒体和质体生物发生与细胞质遗传
线粒体和质体与其它细胞器不同，它们具有自己的遗传物质，同时它们又在很大程度上依赖核基因组。并具有母体遗传的特征，与雄性不育密切相关。本研究组主要研究以下三方面的内容：1）细胞中核基因组、线粒体基因组和质体基因组之间的相互作用；2）质体和叶绿体生物发生的分子遗传调控；3）细胞质遗传与雄性不育。
3. 以拟南芥为模式研究农作物基因组功能
在过去20多年，拟南芥作为模式植物在植物分子生物学，遗传学，植物病理学，次生代谢和发育生物学研究方面得到了非常广泛的应用，其6-8周的生活周期，相对小的基因组，自交，易于遗传转化，非常适合大规模繁殖等特点，使其成为首选的模式植物。该研究组希望建立一套以拟南芥为基础的，快速有效的实验系统，来研究农作物如水稻的基因功能，为农业生产提供技术储备。
研究组成员：
固定人员(Permanent Staff):
唐祚舜博士 助理研究员
Dr. Zuoshun Tang
Assistant Professor
zstang@genetics.ac.cn
石东乔 博士 高级工程师
Dr. Dongqiao Shi
Senior Engineer
dqshi@genetics.ac.cn
刘 洁 工程师
Liu Jie Engineer
jieliu@genetics.ac.cn
研究生：(Graduate Students)
刘乃友 Naiyou Liu, 2002-
刘 巍 Wei Liu, 2002-
于天英 Tianying Yu, 2002-
王思琪 Siqi Wang, 2003-
王 芳 Fang Wang, 2003-
刘 曼 Man Liu, 2003-
袁 黎 Li Yuan, 2003-
李 娜 Na Li, 2004-
鲍佩华 Peihua Bao, 2004-
李红菊 Hongju Li, 2004-
张志峰 Zhifeng Zhang, 2004-
张文娟 Wenjuan Zhang, 2005-
孟钊红 Zhaohong Meng, 2006-
PUBLICATIONS
1. Song, X.F., Yang, C.Y., Liu, J., and Yang, W.C. 2006. RPA, a Class II ARFGAP protein, activates ARF1 and U5 and plays a role in root hair development in Arabidopsis thaliana. Plant Physiology 10.1104/pp.106.07781
2. Ding, Y.H., Liu, N.Y., Tang, Z.S., Liu, J., and Yang, W.C. 2006. Arabidopsis GLUTAMINE RICH PROTEIN23 is essential for early embryogenesis and encodes a novel nuclear PPR motif protein that interacts with RNA polymerase II subunit III. Plant Cell 18: 815-830.
3. Yang, W.-C. 2005. Female Gametophyte Development. In Handbook of Seed Science and Technology (ed. A.S. Basra), Hawthorne Press Inc., New York.
4. Yang, S.L., Jiang, L., Puah, C.S., Xie, L.F., Zhang, X.Q., Chen, L.Q., Yang, W.C., and Ye, D. 2005. Overexpression of TAPETUM DETERMINANT1 alters the cell fates in the Arabidopsis carpel and tapetum via genetic interaction with EXCESS MICROSPOROCYTES1/EXTRA SPOROGENOUS CELLS. Plant Physiology 139: 186-191
5. Shi, D.Q., Liu, J., Xiang, Y.H., Ye, D., Sundaresan, V., and Yang, W.C. 2005. SLOW WALKER1, essential for gametogenesis in Arabidopsis, encodes a WD40 protein involved in 18S ribosomal RNA biogenesis. Plant Cell 17: 2340-2354.
6. Li, X.B., Fan, X.P., Wang, X.L., Cai, L., and Yang, W.-C. The Cotton ACTIN1 gene is functionally expressed in fibers and participates in fiber elongation. Plant Cell 17: 859-875
7. Jiang, J., Yang, S.L., Xie, L.F., Puah, C.S., Zhang, X.Q., Yang, W.C., Sundaresan, V., and Ye, D. 2005. VANGUARD1 encodes a pectin methylesterase that enhances pollen tube growth in the Arabidopsis style and transmitting tract. Plant Cell 17: 584-596
8. Rotman, N., Durbarry, A., Wardle, A., Yang, W.C., Chaboud, A., Faure, J.-F., Berger, F., and Twell, D. 2005. A novel class of MYB factors controls sperm-cell formation in plants. Current Biology 15:244-248
9. Yang, S.L., Xie, L.F., Mao, H.Z., Puah, C.S., Yang, W.C., Jiang, L.X., Sundaresan, V. and Ye, D. 2003. The TAPETUM DETERMINANT 1 gene is required for cell specialization in the Arabidopsis anther. Plant Cell 15, 2792-2804.
10. Wu, X.M. Lim, S.H. and Yang, W.C. 2003. Characterization, expression and phylogenetic study of R2R3-MYB genes in orchid. Plant Mol. Biol. 51: 959-972.
11. Compaan, B., Yang. W.-C., Bisseling, T. and Franssen, H. 2001. ENOD40 expression in the pericycle precedes cortical cell division in Rhizobium-legume interaction and the highly conserved internal region of the gene does not encode a peptide. Plant & Soil, 230:1-8
12. Yang, W.-C. and Sundaresan, V. 2000. Genetics of gametophyte biogenesis in Arabidopsis. Curr Opin Plant Biol. 3:53-57.
13. Yang, W.-C., Ye, D., Xu, J. and Sundaresan, V., 1999. The SPOROCYTELESS gene of Arabidopsis is required for sporogenesis and encodes a novel protein. Genes & Dev. 13:2108-2117.
14. Parinov, S., Sevugan, M., Ye, D., Yang, W.-C., Kumaran, M., Sundaresan, V. 1999. Analysis of flanking sequences from Dissociation insertion lines: a database for reverse genetics in Arabidopsis. Plant Cell, 11:1-9.
15. Yang, W.-C. 1999. Molecular mechanism of root nodule formation. In Molecular Mechanisms of Plant Development (ed. Z.H. Xu and C.M. Liu) (in Chinese). Science Press, Beijing, China.
16. Kumaran, M., Ye, D., Yang, W.-C. and Sundaresan, V. 1999. Molecular cloning of abnormal floral organs: a gene required for flower development in Arabidopsis. Sex. Plant Reprod. 12, 118-122.
17. Heidstra, R., Yang, W. C., Yalcin, Y., Peck, S., Emons, A., Van Kammen, A., and Bisseling, T. (1997). Ethylene provides positional information on cortical cell division but is not involved in Nod factor-induced root hair tip growth in Rhizobium-legume interaction. Development, 124: 1781-1787.
18. Meskiene, I., Yang, W.-C., De Blank, C., Bogre, L., Zwerger, K., Brandstotter, M., Mattauch, M., Bisseling, T. and Hirt, H. 1996. Cell cycle regulation during nodule development. In Biological Fixation of Nitrogen for Ecology and Sustainable Agriculture/NATO ASI series, Series G, Ecological Sciences, 39:63-65.
19. Christiansen, H., Hansen, A.C., Vijn, I., Pallisgaard, N., Larsen, K., Yang W.-C., Bisseling, T., Marcker, K.A. and Jensen, E.O. 1996. A novel type of DNA-binding protein interacts with a conserved sequence in an early nodulin ENOD12 promoter. Plant Mol. Biol., 32: 809-821.
20. Vijn, I., Yang W.-C., Pallisgaard, N., Ostergaard Jensen, E., van Kammen, A., and Bisseling, T. 1995. VsENOD5, VsENOD12 and VsENOD40 expression during Rhizobium-induced nodule formation on Vicia sativa roots. Plant Mol. Biol., 28: 1111-1119.
21. Brito, B., Palacios, J.M., Imperial, J., Ruiz-Argueso, T., Yang, W.-C., Bisseling, T., Schmidt, H., Kerl, V., Bauer, T., Kokotek, W., and Lotz, W. 1995. Temporal and spatial co-expression of hydrogenase and nitrogenase genes from Rhizobium leguminosarum bv. viciae in pea (Pisum sativum L.) root nodules. Mol. Plant-Micobe Interact., 8: 235-240.
22. Vijn, I., Martinez-Abarca, F., Yang, W.-C., das neves, L., van Brussel, A., van Kammen, A., and Bisseling, T. 1995. Early nodulin gene expression during Nod factor-induced processes in Vicia sativa. Plant J., 8: 111-119
23. Bialek, U., Skorupska, A., Yang, W.-C., Bisseling, T., and Van Lammeren, A.A.M. 1995. Disturbed gene expression and bacteroid development in Trifolium pratense root nodules induced by a Tn5 mutant of Rhizobium leguminosaru bv. trifolii defective in exopolysaccharide synthesis. Planta, 197: 184-192.
24. Yang, W.-C., De Blank, C., Meskiene, I., Hirt, H., Bakker, J., Van Kammen, A., Franssen, H., and Bisseling, T. 1994. Rhizobium nod factors reactivate the cell cycle during infection and nodule primordium formation, but the cycle is only completed in primordium formation. Plant Cell, 6: 1415-1426.
25. De Maagd, R.A., Yang, W.-C., Goosen-de Roo, L., Mulders, I.H.M., Roest, H.P., Spaink, H.P., Bisseling, T., and Lugtenberg, B.J.J. 1994. Down-regulation of expression of the Rhizobium leguminosarum outer membrane protein gene ropA occurs abruptly in interzone II-III of pea nodules and can be uncoupled from nif gene activation. Mol. Plant-Microbe Interact., 7: 276-281.
26. Mylona, P., Moerman, M., Yang, W.-C., Gloudemans, T., De Kerkhove, J., Van Kammen, A., Bisseling, T., and Franssen, H.J. 1994. The root epidermis specific pea gene RH2 is homologous to a pathogen related gene. Plant Mol. Biol., 26: 39-50.
27. Matvieko, M., Van de Sande, Yang, W.-C., K., Van Kammen, A., Bisseling, T. and Franssen, H. 1994. Comparison of soybean and pea ENOD40 cDNA clones representing genes expressed during both early and late stages of nodule development. Plant Mol. Biol., 26: 487-493.
28. Yang, W.-C., Katinakis, P., Hendriks, P., Smolders, A., De Vries, F., Spee, J., Van Kammen, A., Bisseling, T., and Franssen, H. 1993. Characterization of GmENOD40, a gene showing novel patterns of cell-specific expression during soybean nodule development. Plant J., 3: 573-585.
29. Yang, W.-C., and Bisseling, T. 1993. Nodulin gene expression during pea nodule development. In Current Developments in Soybean-Rhizobium Symbiotic Nitrogen Fixation (ed. X. Dou). Heilongjiang Science & Technology Publishing House, Harbin, China, pp55-62
30. Kardailsky, I., Yang, W.-C., Zalensky, A., Van Kammen, A., and Bisseling, T. 1993. The pea late nodulin gene PsNOD6 is homologous to the early nodulin genes PsENOD3/14 and is expressed after the leghaemoglobin genes. Plant Mol. Biol., 23: 1029-1037.
31. Franssen, H., Yang, W.-C., Katinakis, P. and Bisseling, T. 1993. Characterization of GmENOD40, a gene expressed in soybean nodule primordia. Curr. Plant Sci. Biotechnol. Agric. 17:275-284.
32. De Blank, C., Mylona, P., Yang, W.-C., Katinakis, P., Bisseling, t., and Franssen, H. 1993. Characterization of the soybean early nodulin cDNA clone GmENOD55. Plant Mol. Biol., 22: 1167-1171.
33. Franssen, H., Heidstra, R., Geurts, R., Horvath, B., Moerman, M., Yang, W.-C., Vijn, I. And Bisseling, T. 1993. Nodulin genes as molecular markers to study Rhizobium nod factor activity. Plant Signals in Interactions with Other Organisms (Penn. State Symposium in Plant Physiology: Current Topics in Plant Physiology), 11:164-170.
34. Yang, W.-C., Canter Cremers, H.C.J., Hogendijk, P., Katinakis, P., Wijffelman, C.A., Franssen, H., Van Kammen, A., and Bisseling, T. 1992. In-situ localization of chalcone synthase mRNA in pea root nodule development. Plant J., 2: 143-151.
35. Franssen, H., Vijn, I., Yang, W.-C., and Bisseling, T. 1992. Developmental aspects of the Rhizobium-legume symbiosis. Plant Mol. Biol., 19: 89-107.
36. Yang, W.-C., Horvath, B., Hontelez, J., Van Kammen, A., and Bisseling, T. 1991. In situ localization of Rhizobium mRNAs in pea root nodules: nifA and nifH localization. Mol. Plant-Microbe Interact. 4: 464-468.
37. Franssen, H., Scheres, B., Van De Wiel, C., Horvath, B., Moerman, M., Yang, W.-C., Govers, F. And Bisseling, T. 1990. Nodulins and nodule development. In Nitrogen Fixation: Achievements and Objectives (ed. P.M. Gresshoff). P709-712.
