凌宏清 研究员
职务：植物细胞与染色体工程国家重点实验室主任
Emailt:hqling@genetics.ac.cn
个人简介
凌宏清 男，博士，研究员，博士生导师。
凌宏清博士，研究员，植物细胞与染色体工程国家重点实验室主任。
1982年获四川农业大学农学院学士, 1988年获德国基尔市Christian-Albrechts大学农学院农学硕士学位, 1993年获德国基尔市Christian-Albrechts大学理学院理学博士，同年进入联邦德国植物遗传与栽培作物研究所从事博士后研究，1998年瑞士苏黎市大学植物生物学所教授高级助理。2000年入选中国科学院国外引进杰出人才"百人计划"。2001年回国，中国科学院遗传与发育生物学研究所研究员，创新课题组长。获得2002年度"国家杰出青年基金"。2004年入选国家7部委（人事部、科学技术部、教育部、财政部、国家发展和改革委员会、国家自然科学基金委员会、中国科学技术协会"新世纪百千万人才工程"国家级人选。
主要研究方向
植物营养分子生物学、植物根功能基因组学及小麦抗病基因的分离。
主要研究内容
1. 植物营养分子生物学
植物的生长发育需要十多种矿质营养元素。任何一种必需元素的缺乏将影响植物的生长发育，最终导致产量和品质下降。氮、磷、铁是世界范围内三大主要限制农作物生长发育的矿质营养元素。创新研究组的工作重点是研究植物从土壤中对磷、铁两种矿质元素的吸收代谢分子生物学；分离与磷、铁吸收代谢相关的基因并阐明它们在控制磷、铁元素吸收代谢中的机理。在铁元素吸收方面，用图位克隆法从西红柿基因组中分离出了控制铁元素吸收代谢的两个关键基因（chloronerva and fer），目前正在进行分子生物学方面的研究。在磷元素的研究中，正进行pds基因的图位克隆。该研究得到中国科学院“百人计划”和“十.五”国家863课题资助。
2. 根功能基因组学
根是植物生长发育的基础。然而，由于植物根部的研究难度大，再加上根部功能基因致死突变体在模式植物拟南芥和水稻中难以保存，极大地限制了根部重要功能基因的研究。西红柿是一个二倍体栽培作物，基因组相对较小（950 Mb），有很好的细胞和分子遗传学研究基础，并已建起了一套非常有效的图位克隆分离基因体系。与水稻和拟南芥相比，以西红柿为模式植物研究根功能基因的最大优点是易嫁接，根功能基因缺陷型致死突变体可通过嫁接得以保存和繁殖。创新研究组应用化学和物理两种方法处理西红柿种子，建立突变群体，筛选根部功能基因致死型突变体，嫁接到野生型植株，加以保存和繁殖。力求建立一个较完整的根功能基因缺陷致死型突变体文库，用于根功能基因组学研究。该项目得到中国科学院“知识创新工程领域前沿项目”资助。
3. 小麦抗病基因的分离
小麦生产受到各种病虫害的威胁。小麦叶锈病是小麦生产中最为普遍的一种病害，其流行将严重影响小麦产量和品质。创新研究组选择小麦叶锈病第一抗病基因为目的基因从事小麦图位克隆。已建立一套有效的小麦大群体单株DNA分析方法。并将目的基因LR1精确定位于小麦5D染色体末端的两个紧密连锁的RFLP markers之间, 两个RFLP标记之间的物理距离大约为250 kb 左右。染色体步移目前正在进行之中。LR1基因的克隆将建立一套适用于小麦的图位克隆方法，为有效地分离小麦重要农艺性状基因提供技术平台。通过对小麦叶锈病第一抗病基因的分离及分子生物学研究，将为培育新的小麦叶锈病抗病品种提供理论基础。此研究得到国家自然科学基金的资助。
KEY PUBLICATIONS
Zhao, T., H.-Q. Ling: Effects of pH and nitrogen forms on expression profiles of genes involved in iron homeostasis in tomato. Plant Cell and Environment, doi: 10.1111/j.1365-3040.2007.01638 (2007)
Feng, Z.-Y., X.-J. Liu, Y.-Z. Zhang, H.-Q. Ling: Genetic diversity of Tibetan wild barley using SSR markers. Acta Genetica Sinica 33(10): 917-928 (2006)
Chen, Y.-H., X.-M. Wu, H.-Q. Ling, W.-C. Yang: Expression of DwMYB2 impaired iron transport from root to shoot in Arabidopsis thaliana. Cell Research 16: 830-840 (2006)
Yan, X., P. Wu, H.-Q. Ling, G.. Xu, F. Xu, Q. Zhang: Plant nutriomics in China-an overview. Annual of Botany 98：473-482 (2006)
Feng, Z.-Y., L.-L. Zhang, Y.-Z. Zhang, H.-Q. Ling: Genetic diversity and geographical differentiation of cultivated six-rowed naked barley landraces from the Qinghai-Tibet plateau of China detected by SSR analysis. Genetics and Molecular Biology 29 (2): 330-338 (2006)
Feng, H., F. An, S. Zhang, Z. Ji, H.-Q. Ling, J. Zuo: Light-regulation, tissue- and cell differentiation-specific expression of the Arabidopsis Fe(III)-chelate reductase gene AtFRO6. Plant Physiology 140: 1345-1354 (2006)
Zhang, J., H.-F. Zhu, H. Liang, K.-F. Liu, A.-M., Zhang, H.-Q. Ling, D.-W. Wang: Further analysis of the function of AtbHLH29 in regulating the iron uptake process in Arabidopsis thaliana. Journal of Integrative Plant Biology 48(1): 75-84 (2006)
Wu, H., L. Li, J. Du, Y. Yuan, X. Cheng, H.-Q. Ling: Molecular and biochemical characterization of Fe(III)-chelate reductase gene family in Arabidopsis thaliana. Plant Cell Physiology 46 (9): 1505-1514 (2005)
Yuan, Y.X, J. Zhang, D.W. Wang, H.-Q. Ling: AtbHLH29 of Arabidopsis thaliana is a functional ortholog of tomato FER involved in controlling iron acquisition in strategy I plants. Cell Research 15(8): 613-621 (2005)
Guyot, R., X. Cheng, Y. Su, Z. Cheng, E. Schlagenhauf, B. Keller, H.-Q. Ling: Complex organization and evolution of the tomato pericentromeric region at the FER gene locus. Plant Physiology 138: 1205-1215 (2005)
Mueller，L.A., S.D.Tanksley, J.J.Giovannoni, J. van Eck, S. Stack, D. Choi, B.D. Kim, M. Cheng, C. Li, H.-Q. Ling, Y. Xue, G. Seymour, G. Bishop, G. Bryan, R. Sharma, J. Khurana, A. Tyagi, D. Chattopadhyay, N.K. Singh, W. Stiekema, P. Lindhout, T. Jesse, R. K. Lankhorst, M. Bouzayen, D. Shibata, S. Tabata, A. Granell, M. A. Botella, G. Giuliano, L. Frusciante, M. Causse, D. Zamir: The tomato sequencing project, the first cornerstone of the International Solanaceae Project (SOL). Comp Funct Genom 6: 153-158 (2005)
Ling, H.-Q., L. Li, Y. Yuan and T. Zhao: Molecular regulation mechanism of iron uptake in tomato. In: Plant Nutrition for Food Security, Human Health and Environmental Protection, eds by C. J. Li et al. Tsinghua University Press, pp 92-93 (2005)
Feng, Z., L. Zhang, Y. Zhang, H.-Q. Ling: Further molecular evidence for the hordeum vulgare ssp. spontaneum in Tibet as ultimate progenitor of Chinese cultivated barley. High Technology Letters 11 (3): 320-324 (2005)
Li, L., X. Cheng, H.-Q. Ling: Isolation and characterization of Fe(III)-chelate reductase gene LeFRO1 in tomato. Plant Mol. Biol 54: 125-136 (2004)
Ling, H.-Q., J. Qiu, R.P. Singh, B. Keller: Identification and genetic characterization of an Aegilops tauschii ortholog of the wheat leaf rust disease resistance gene Lr1. Theor. Appl. Genet. 109: 1133-1138 (2004)
Li, C., G. Liu, C. Xu, G. I. Lee, P. Bauer, H.-Q. Ling, M. W. Ganal and G. A. Howe: The tomato suppressor of prosystemin-mediated responses2 gene encodes a fatty acid desaturase required for the biosynthsis of jasmonic acid and the production of a systemic wound signal for defense gene expression. Plant Cell 15: 1646-1661 (2003)
Ling, H.-Q., Y.X. Zhu and B. Keller: High-resolution mapping of the leaf rust disease resistance gene Lr1 in wheat and physical walking to the gene using a BAC library of Aegilops tauschii. Theor. Appl. Genet. 106: 875-882 (2003)
Ling, H.-Q., and B. Keller: Genetic transformation of Linum species. In: Plant Genetic Engineering Vol 4: Improvement of Commercial Plants-II. Ed by Pawan K Jaiwal and Rana P. Singh. Sci Tech Publishing LLC, U.S.A. pp235-260 (2003)
Ling, H.-Q., P. Bauer, B. Keller, M. Ganal: The fer gene encoding a bHLH transcriptional regulator controls development and physiology in response to iron in tomato.Proc. Natl. Acad. Sci. USA, vol. 99, 13938-13943 (2002)
Ling, H.-Q. and M. W. Ganal: Towards map-based cloning of two genes involved in iron uptake of tomato. Journal of Plant Nutrition 23: 1953-1967 (2000).
Ling, H.-Q., G.. Koch, H. Baeumlein, M. W. Ganal: Map-based cloning of chloronerva - a gene involved in iron uptake of higher plants encoding nicotianamine synthase. Proc. Natl. Acad. Sci. USA, Vol. 96, 7098-7103 (1999)
Ling, H.-Q., D. Kriseleit, M. W Ganal: Effect of ticarcillin/potassium clavulanate on callus growth and shoot regeneration in Agrobacterium tumefaciens-mediate transformation of tomato (Lycopersicon esculentum). Plant Cell Reports 17, 843-847 (1998).
Ganal, M.W., R. Czihal, U. Hannappel, D. Kloos, S. K?nig, H.-Q. Ling: Sequencing of cDNA clones from the genetic map of tomato (Lycopersicon esculentum). Genome Res. 8: 842-847(1998).
Ling H.-Q., H. Binding:Transformation in protoplast cultures of Linum usitatissimum and L. suffruticosum mediated with PEG and with Agrobacterium tumefaciens. J. Plant Physiol.151: 479-488 (1997).
Ling H.-Q., A. Pich, G. Scholz and M.W. Ganal: Genetic analysis of two tomato mutants affected in the regulation of iron methabolism. Mol. Gen. Genet. 252: 87-92 (1996).
