吴中柳 中国科学院成都生物研究所 研究员
联系电话：028-85221263
电子邮件：wuzhl@cib.ac.cn
吴中柳研究员，女，1975年6月生。2003年毕业于中国科学院上海有机化学研究所，获理学博士学位，同年赴美国Vanderbilt大学医学院从事博士后研究工作，2006年入选中国科学院“百人计划”(“引进国外杰出人才”) ，现任中国科学院成都生物研究所应用与环境微生物研究中心研究员，博士生导师。主要从事生物催化与生物转化研究。
研究内容：
1. 根据化合物物化性质，设计新型高通量筛选策略；运用微生物宏观基因组学，从环境中筛选新型酶源，构建催化性能各异的储备酶库。
2. 利用分子生物学、化学或物理的手段，提高酶催化剂的活力和立体选择性，扩展其底物适应性，改善其环境耐受性。
3.结合有机合成设计，发展在医药和精细化工领域有重要意义的手性分子的酶催化合成新方法。
4.运用外源表达的单氧化酶细胞色素P450，进行新药的药理毒理预筛和代谢评估。目前已在国际重要刊物上发表论文十余篇，论文被他人引用近百次，申请国内发明专利三项。
主要代表论著：
1. Wu, Z.-L.; Sohl, C.D.; Shimada, T.; Guengerich, F.P. Recombinant Enzymes Over expressed in Bacteria Show Broad Catalytic Specificity of Human Cytochrome P450 2W1 and Limited Activity of Human Cytochrome P450 2S1. Mol. Pharmacol., 2006, 69, 2007-2014.
2. Wu, Z.-L.; Bartleson, C.J.; Ham, A.J.; Guengerich, F.P. Heterologous Expression, Purification, and Properties of human cytochrome P450 27C1, Arch.. Biochem. Biophys. 2006, 445, 138-146.
3. Wu, Z.-L.; Podust, L.M.; Guengerich, F.P. Expansion of Substrate Specificity of Cytochrome P450 2A6 by Random and Site-Directed Mutagenesis. J. Biol. Chem. 2005, 280, 41090-41100.
4. Kim, D.; Wu, Z.-L.; Guengerich, F.P. Analysis of coumarin 7-hydroxylation activity of cytochrome P450 2A6 using random mutagenesis. J. Biol. Chem. 2005, 280, 40319-40327.
5. Wu, Z.-L.; Aryal, P.; Lozach, O.; Meijer, L.; Guengerich, F.P. Biosynthesis of New Indigoid Inhibitors of Protein Kinases Using Recombinant Cytochrome P450 2A6, Chem. Biodiv. 2005, 2(1), 51-65.
6. Guengerich, F.P.; Wu, Z.-L.; Bartleson, C.J. Function of Human Cytochrome P450s: Characterization of the Orphans. Biochem. Biophys. Res. Commun. 2005, 338, 465-469.
7. Guengerich, F.P.; Wu, Z.-L. Biosynthesis of novel indirubins by recombinant cytochrome P450 systems. Invited chapter in Indirubin, the Red Shade of Indigo, edited by Meijer, L.; Guyard, N.; Skaltsounis, L; Eisenbrand, G. Editions , Roscoff, Fr. 2006.
8. Wu, Z.-L.; Li, Z.-Y. Highly Enantioselective Synthesis of a,a-Disubstituted Malonamic Acids through Asymmetric Hydrolysis of Dinitriles with Rhodococcus sp. CGMCC 0497, Chem. Comm. 2003, 3, 386-387.
9. Wu, Z.-L.; Li, Z.-Y. Practical Synthesis of Optically Active a,a-Disubstituted Malonamic Acids through Asymmetric Hydrolysis of Malonamide Derivatives with Rhodococcus sp. CGMCC 0497, J. Org. Chem. 2003, 68(6), 2479-2482.
10. Wu, Z.-L.; Li, Z.-Y. Biocatalytic Asymmetric Hydrolysis of (±)-b-Hydroxy Nitriles by Rhodococcus sp. CGMCC 0497, J. Mol. Catal. B: Enzym. 2003, 22(1-2), 105-112.
11. Wu, Z.-L.; Li, Z.-Y. Enantioselective Biotransformation of a,a-Disubstituted Dinitriles to the Corresponding 2-Cyano-Acetamides using Rhodococcus sp. CGMCC 0497, Tetrahedron: Asymmetry, 2003, 2133-2142.
12. Wu, Z.-L.; Li, Z.-Y. Enhancement of Enzyme Activity and Enantioselectivity via Cultivation in Nitrile Metabolism by Rhodococcus sp. CGMCC 0497, Biotechnol. Appl. Biochem. 2002, 35, 61-67.
13. Wu, Z.-L.; Li, Z.-Y. Enantioselective Hydrolysis of Various Racemic *-Substituted Arylacetonitriles using Rhodococcus sp. CGMCC 0497, Tetrahedron: Asymmetry 2001, 12, 3305-3312.
