阮康成
研究员，分子生物学国家重点实验室开放研究组组长，博士生导师
电话：021-54921268
E-mail: kcruan@sibs.ac.cn
个人简介：
1946年9月生。1969年毕业于北京大学化学系，1981年上海生物化学研究所研究生毕业，获理学硕士学位。1985年起曾多次赴美国Illinois大学生化系G·Weber教授实验室进行合作研究，1995年起多次赴法国INSERM进行合作研究。现任中国生物物理学会第七届理事会副理事长，上海市生物物理学会第六届理事会副理事长。一直从事利用荧光光谱学等研究生物大分子的结构、功能和相互作用，蛋白质芯片等。在国内首先将毫微秒荧光光谱学应用于蛋白质的研究，揭示了人异常血红蛋白HbM(I)分子构象和异常功能的关系。研究并建立了利用荧光探针快速测定RNA序列、检测糖蛋白的方法。从八十年代末开始将高压力方法引入生物大分子的研究，利用高压力研究了甘油醛-3-磷酸脱氢酶、磷酸化酶等一系列寡聚酶的解离和重组，trypsin, 33kDa等蛋白质的folding/unfolding。证实了conformational drifted form的存在，首次提出了多亚基寡聚酶在构象、自由能等方面的heterogeneity模型；证实了由亚基到寡聚酶是由一熵驱动的自发过程等。用实验方法首次证明磷酸化酶a、b的亚基可以杂交以及相同亚基组成的磷酸化酶a四聚体中亚基间interface的ΔV和结合自由能是不对称的；与G. Weber建立了研究ligand与蛋白质、酶相互作用的方法；研究鸡法氏囊病病毒IBDV时发现在一定条件下、经一定压力处理后病毒不可逆地失去全部感染性，但保持原有免疫特性，表明高压力可成为一种全新的制备病毒疫苗方法，为进一步研究病毒作用及其免疫机制提供了新途径。发现sucrose等对蛋白质的稳定作用除了和Timashiff提出的增加自由能有关外，还与蛋白质标准体积变化的减少有关。在近来的蛋白质芯片的研究中，亦取得了可喜的进展。在国内外学术杂志上共发表研究论文80余篇。曾获中科院重大科技成果二等奖。共获国内专利一项，申请国内专利二项。
研究方向：生物大分子结构功能和作用
研究工作：
主要利用动态、稳态荧光光谱学及其他光谱学方法研究蛋白质结构-功能，蛋白质分子间以及蛋白质分子与其他生物大子的相互作用；量子点在分子生物学、细胞生物学研究中的重大应用；蛋白质芯片的研究。
发表论文：
1. Cui-Yan Tan, Chun-He Xu, Jun Weng, Jian-Ren Shen, Shinsuke Sakuma, Yasusi Yamamoto, Reinhard Lange, Claude Balny and Kang-Cheng Ruan. Pressure Equilibrium and Jump Study on Unfolding of 23kDa Protein from Spinach Photosystem II. Biophysical Journal, 2005, 88 (2):1264-1275
2. Ru-Qiang Liang, Wei Li, Yang Li, Cui-yan Tan, Jian-Xun Li, You-Xin Jin, Kang-Cheng Ruan. An oligonucleotide microarray for microRNA expression analysis based on labeling RNA with quantum dot and nanogold probe. Nucleic Acids Research, 2005, 33 (2):e17
3.Feng Zhang, Jinpeng Gao, Jun Weng, Cuiyan Tan, Kangcheng Ruan, Chunhe Xu, and Dean Jiang. Structural and functional differentiation of three groups of tyrosine residues by acetylation of N-acetylimidazole in manganese stabilizing protein. Biochemistry, 2005, 44:719-725
4.T.-F. CHANG and K.-C. RUAN. Exploration of pressure-induced dissociation of pyruvate oxidase. Cell. Mol. Biol., 2004, 50 (4):323-328
5.Hui-Zhi WANG, Hai-Yan WANG, Ru-Qiang LIANG, Kang-Cheng RUAN. Detection of tumor marker CA125 in ovarian carcinoma using quantum dots. Acta Biochimica et Biophysica Sinica, 2004, 36(10):681-686
6.WENG Jun, TAN Cuiyan, YU Yong, RUAN Kangcheng & XU Chunhe. Low pH-inducedconformational changes in 33 kD protein of photosystem II. Chinese Science Bulletin, 2004, 49(9):921-925
7.Jun Weng, Cuiyan Tan, Jian-Ren Shen, Yong Yu, Xiaomei Zeng, Chunhe Xu, and Kangcheng Ruan. pH-induced conformational changes in the soluble manganese-stabilizing protein of photosystem II. Biochemistry, 2004, 43:4855-4861
8.Ru-Qiang Liang, Cui-Yan Tan, and Kang-Cheng Ruan. Colorimetric detection of protein microarrays based on nanogold probe coupled with silver enhancement. Journal of Immunological Methods, 2004, 285:157-163
9.RUAN Kang-Cheng. Fluorescence spectroscopy of quantum dot in biological sciences. ChineseBulletin of Life Sciences, 2003, 15(2):84-87
10.Kangcheng Ruan, Chunhe Xu, Tingting Li, Jiong Li, Reinhard Lange and Claude Balny. The thermodynamic analysis of protein stabilization by sucrose and glycerol against pressure-induced unfolding--The typical example of the 33-kDa protein from spinach photosystem II. Eur. J. Biochem., 2003, 270:1654-1661
11.Chunhe Xu, Kangcheng Ruan, Yong Yu and Jun Weng. Studies on the molecular mechanism of 33 kDa extrinsic protein in photosystem II oxygen-evolving complex. Journal of Photoscience, 2002, 9(2):82-85
12.Kang cheng Ruan, Jiong Li, Ruqiang Liang , Chunhe Xu, Yong Yu, Reinhard Lange and Claude Balny. A rare protein fluorescence behavior where the emission is dominated by tyrosine. Case of the 33-kDa protein from spinach photosystem II. Biochemical and Biophysical Research Communications, 2002, 293:593-597
13.Kangcheng Ruan and Claude Balny. High pressure and static fluorescence to study macromolecular structure-function. Biochimica et Biophysica Acta, 2002，1595:94-102
14.TAN Cui-Yan, LIANG Ru-Qiang, RUAN Kang-Cheng. Application of quantum dot to life science. Acta Biochimica et Biophysica Sinica, 2002, 34(1):1-5
15.Qiaoqiao Ruan, Kangcheng Ruan, Claude Balny, Michael Glaser and William W. Mantulin. Protein folding pathways of adenylate kinase from E.coli: Hydrostatic pressure and stopped-flow studies. Biochemistry, 2001, 40:14706-14714
16.Kangcheng Ruan, Chunhe Xu, Yong Yu, Jiong Li, Reinhard Lange, Nicole Bec and Claude Balny. Pressure-exploration of the 33-kDa protein from spinach photosystem II particle. Eur. J. Biochemistry, 2001, 268:2742-2750
17.Xiang Gao, Kang-Cheng Ruan and Wang-Yi Liu. Exploration of RNA 3′ terminal locations in rat ribosome. Mol.Cell.Biochem., 2001, 225:161-165
18.Shuang Tang, Liang Xie, Fa-Jian Hou, Wang-Yi Liu, Kang-Cheng Ruan. Non-specific deadenylation and deguanylation of naked RNA catalyzed by ricin under acidic condition. Biochim. Biophys. Acta, 2001, 1519: 192-198
19.Y. YU, S.-M. TIAN, K.-C. RUAN, and C.-H. XU. The release of extrinsic polypeptides and manganese cluster from photosystem 2 membranes under high hydrostatic pressure. Photosynthetica, 2001, 39(1):115-117
20.Yong Yu, Rong Li, Chunhe Xu, Kangcheng Ruan, Yunkang Shen and Govindjee. N-Bromosuccinimide modification of tryptophan 241 at the C-terminus of the manganese stabilizing protein of plant photosystem II influences its structure and function. Physiologia Plantarum, 2001, 111:108-115
21.Shuang Tang, Rong-gui Hu, Wang-yi Liu and Kang-cheng Ruan. Non-specific depurination activity of saporin-S6, a ribosome-inactivating protein, under acidic conditions. Biol. Chem., 2000, 381:769-772
22.HU Liang-Yan, TIAN Shao-Min, YE Qi-Zhuang, RUAN Kang-Cheng. Comparison of the catalytic domains of collagenase-1 and stromelysin-1. Acta Biochimica et Biophysica Sinica, 2000, 32(4):409-412
23.Shao-Min Tian, Kang-Cheng Ruan, Jian-Fei Qian, Guo-Qing Shao and Claude Balny. Effects of hydrostatic pressure on the structure and biological activity of infectious bursal disease virus. Eur. J. Biochemistry, 2000, 267:4486-4494
24.Kangcheng Ruan, Shaomin Tian, Reinhard Lange and Claude Balny. Pressure effects on tryptophan and its derivatives. Biochemical and Biophysical Research Communications, 2000, 269:681-686
25.Kangcheng Ruan, Reinhard Lange, Filip Meersman, Karel Heremans and Claude Balny. Fluorescence and FTIR study of the pressure-induced denaturation of bovine pancreas trypsin. Eur. J. Biochemistry, 1999, 265:79-85
